p53蛋白及其突变体对RhoE基因转录调控的影响

doi:10.3969/j.issn.1004-616x.2012.05.002

›› 2012, Vol. 24 ›› Issue (5): 330-334.doi: 10.3969/j.issn.1004-616x.2012.05.002

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The effect of the transcriptional regulation of p53 on RhoE gene

ZHU Ya-jie1;SU Xiao-mei1;CHENG Peng1;ZHANG Tao1;BI Feng

(1. Department of Oncology, General Hospital of Chengdu Military Region, Chengdu 610083; 2. Department of Oncology, West China School of Medicine, Sichuan University, Chengdu 610041, Sichuan, China)

Received:2012-03-09 Revised:2012-05-29 Online:2012-09-30 Published:2012-09-30
Contact: zhuyajie 112891@126.com

Abstract

Abstract: OBJECTIVE: p53 gene is the most important human tumor suppressor gene. The purpose of this study was to explore the effect of p53 and its mutants on the transcriptional regulation of RhoE. METHODS：We constructed PEGFP-wt-p53 plasmid，and then synthesized p53 mutation hotspot plasmid (single point and double point mutations) by site-directed mutagenesis PCR technology. pGL3-RhoE-promotor-Luc plasmid transiently transfected the wild-type and mutant p53 plasmid into PC3 (p53-null) cells. Then the transcription activities of p53 and its mutants on RhoE were assessed by Dual-Luciferase reporter assay，and RhoE protein expression analyzed by western-blot. RESULTS：p53 protein could regulate the transcription activity of RhoE (P<0.05)，but the mutants lost this function. The effect of the different p53 mutants on RhoE transcription regulation showed no significant difference (P>0.05). The results was confirmed by western-blot on protein level. CONCLUSION：RhoE is one of the p53 regulated genes，but once p53 mutated，it would not able to regulate the transcription activity of RhoE anymore.

Key words: p53 protein, RhoE, mutation, transcription

ZHU Ya-jie1;SU Xiao-mei1;CHENG Peng1;ZHANG Tao1;BI Feng. The effect of the transcriptional regulation of p53 on RhoE gene[J]. , 2012, 24(5): 330-334.

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The effect of the transcriptional regulation of p53 on RhoE gene

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